A set of 36 LGL GST-fusion fragments was cloned (USER system, New England Biolabs) and screened for solubility by an enzyme-linked immuno- sorbent assay. Three soluble fragments with high expression were purified from protein gels, used for injection of rabbits (Charles River Laboratories, France), and tested in worms. IgG from two sera were further used and affinity purified by corresponding MBP-fusion protein columns (primers of fragments used were forward 50 ggggagcuTTAATATGCACAAAATGCGA GAT30 , reverse 50 ggggaacuTATTTGATAACCCGCATTGTAC30 and forward 50 ggggagcuCGCACTTCATTGGAGTTTGAC30 , reverse 50 ggggaacuTCGCTT TGGACTTGTCTCATG30 ).
lgl-1