Phospho-specific antibodies against S11 in HTP-1 were produced by injecting rabbits with the synthetic peptide (DEpSLNKSADSIDDKC) (GenScript). Specificity of the antibodies was validated by absence of staining in germlines of worms expressing a htp-1S11A transgene in a htp-1 (gk174), htp-2 (tm2543) mutant background.
htp-1