Polyclonal antisera against a bacterially expressed APH-2-fusion protein. Affinity-purified antisera from each of three different rabbits stain wild-type embryos in a similar manner, but show no staining of aph-2 mutant embryos. aph-2
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). csc-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). bub-1