WBAntibody00001765

Rabbit polyclonal peptide antibody that stains all neurons in C.elegans.

WBAntibody00000095

MAb219, mouse monoclonal antibody that recognize a highly conserved epitope in all pgp isoforms. pgp-1
pgp-3

WBAntibody00000327

Polyclonal rabbit antibody against recombinant protein. Anti-NID-1 antiserum that reacts with all isoforms. nid-1

WBAntibody00001756

Monoclonal antibody against tubulin, non-worm antibody, but react with all worm microtubules with bright staining.

WBAntibody00001914

Antibody that cross-reactive with all H1 variants except H1.X, with strong reactivity for HIL-4. hil-4

WBAntibody00000353

Rabbit polyclonal antiserum was raised against a region common to all isoforms and used to localize the protein in whole animals. cle-1

WBAntibody00002170

Monoclonal antibodies against C. elegans SNB-1, without the transmembrane region were made by immunizing mice with a His6-tagged fusion protein to amino acids 1-86 which had previously been used for the generation of rabbit polyclonal antibodies. Three stable hybridoma cell lines (2E6, 5D1, and 5G1) were produced and all were of the isotype IgG1. snb-1

WBAntibody00003048

Human SPOP is highly homologous to C. elegans SPOP-1 with 65.9% amino acid homology. We raised monoclonal antibodies against human SPOP by producing recombinant SPOP as a 6×His tagged protein in E. coli and used the purified full length SPOP protein as an immunogen to inject naïve BALB/C mice. All mAb production work was conducted at the Washington State University Monoclonal Antibody Center as a fee for service project. Human SPOP reactive mAb clones were screened for reactivity against C. elegans SPOP-1 protein, and clone 8B was selected for its specificity. spop-1

WBAntibody00002841

Full-length RPL-5, RPL-25.2, RPS-3 and RPS-0 ribosomal proteins tagged with six Histidine residues (RPL-5-His6, RPL-25.2-His6, RPS-3-His6, RPS-0-His6) were expressed in E. coli. The recombinant proteins were purified by Ni-NTA agarose beads and the eluted RPL-5-His6, RPS-3-His6 and RPS-0-His6 proteins were used to raise polyclonal antibodies in rat. For RPL-25.2-His6, a specific protein band was excised from the SDS-PAGE and used to generate polyclonal antibody in rat. All polyclonal antibodies were generated in the Antibody Center at NIBS (National Institute of Biological Sciences, Beijing, China). rpl-25.2

WBAntibody00000361

Mouse monoclonal antibody against UNC-1 recombinant protein. Antibodies to UNC-1 were prepared in the laboratory of Man Sun Sy. Two mice were inoculated three times with UNC-1 protein. Once a response was documented from the serum of the mice, hybridomas were made and screened for anti-UNC-1. Seven monoclonal antibodies reacting with UNC-1 were isolated. Each antibody was screened for the ability to bind UNC-1 on a Western blot and for immunocytochemical staining in N2, as well as loss of staining in the unc-1 null alleles fc53 and e580. Five monoclonal antibodies specific for UNC-1 were isolated, each of which gave identical binding in nematodes and on Western blots. One monoclonal antibody, 5D5, was used for all the studies. unc-1