WBAntibody00000424

Monoclonal human antibody produced via phage library screen. An antibody that specifically binds to the centrosome of C. elegans cells was isolated.

WBAntibody00000448

Polyclonal rabbit peptide antibody against PAR-5. A peptide, VGAEDQEQEGNQEAGN, representing amino acids 233248 of the predicted PAR-5 protein was coupled via a cysteine residue at the N terminus to keyhole limpet haemocyanin via m-maleimidobenzoyl-N-hydroxysuccinimide ester and used to raise polyclonal antibodies in rabbit. The antibody was affinity-purified against a GST fusion of full-length PAR-5 protein. par-5

WBAntibody00002913

Rabbit antisera to ECP-1 was raised with the synthetic peptide CEVHLHKFLDRDYRIK (residues 571-585 of ECP-1), coupled to hemocyanin via the extra N-terminal cysteine. ifc-2

WBAntibody00000425

Monoclonal human antibody produced via phage library screen. The zinc finger domain of POS-1 corresponding to the 90th179th amino acid residues was used as an antigen. pos-1

WBAntibody00002874

polyclonal rabbit anti-FAHD-1 antibody produced by BioGenes GmbH, Berlin, Germany, using full-length His-tagged ceFAHD-1 protein produced in chick cells for immunization; purified in our lab from serum via NHS-column fahd-1

WBAntibody00000385

Polyclonal rabbit peptide antibody against IFB-1. A rabbit B1 antiserum was raised with the synthetic peptide YSIPANVVIKPGKNL (residues 489503 of B1) as antigen. The peptide was coupled to hemocyanin via an extra N-terminal cysteine and used for antigen-affinity purification of the B1 antiserum. ifb-1

WBAntibody00002726

For NTL-1, the region corresponding to amino acid position 650-950 was amplified from cDNA using forward primer: 5'-ATAATAGGATCCAGGTAATGAAAGAGAACTCGG-3' (Tdo1707); and reverse primer: 5' -TATTATGGATCCCAAATTTTCCACTGACATCGC-3' (Tdo1708) and cloned into pCAL-KC via BamHI/BamHI. This construct was used as a template for generating the antigen for mouse polyclonal against NTL-1 (4162, Capralogics Inc.). let-711