To produce antibodies against the NES like sequence of SOD1, a SOD1 peptide corresponding to residues 33-51 (GSIKGLTEGLHGFHVHEFGC) was synthesized by Peptide 2.0 Inc. (Chantilly, VA). A Cysteine was added to the C-terminus for conjugation of the peptide to keyhole limpet hemocyanin (KLH) to improve the immunogenicity. Anti-NLP was purified from rabbit antiserum using the same peptide that was biotinylated at the C-terminus and bound to streptavidin magnetic beads (Thermo scientific, Waltham, MA).
sod-1