PLK-1 antibody was generated against a peptide corresponding to the amino-terminal 20 amino acids of the PLK-1 by conjugation to keyhole limpet hemocyanin and injection into rabbit. plk-1
BL52/B6 mice were subcutaneously primed with 100 μg FIC-1 in PBS supplemented with complete Freund's adjuvant, boosted by intraperiotenal injection 4 weeks later with 20 μg in PBS and terminally bled 5 days later. fic-1
Polyclonal antibody was raised in rabbits against a peptide corresponding to the first extracellular loop of CLC-1 (20 residues long) coupled to Keyhole limpet hemocyanin and was affinity purified with GST fusion proteins with full-length CLC-1. clc-1