Polyclonal antisera were raised to anti-NGAPQPFVRFamide peptide encoded by flp-32 in guinea pigs (Genosphere Biotechnologies, France), N-terminally coupled to keyhole limpet hemocyanin (KLH) and affinity purified. flp-32
A specific rabbit antiserum raised against the carboxy-terminal part of the CEH-32 protein was used in immunostaining experiments to confirm and complete the expression pattern. The specificity of this antibody was demonstrated by lack of staining after preincubation with the immunizing antigen. ceh-32