Antibodies against PRDE-1 and TOFU-5 were produced at the Laboratory Animal Resources (European Molecular Biology Laboratory, Heidelberg). For this, New Zealand White female rabbits were immunized with the recombinant full-length proteins together with complete Freund's adjuvant. After the priming immunization, four further booster immunizations were performed every 3 wk. The harvested serum was purified by affinity purification using the recombinant full-length PRDE-1 or TOFU-5, respectively.
pre-1