Rabbit WSP-1 polyclonal antiserum was raised against amino acids 475 to 598, corresponding to the VCA region of WSP-1. The antigen was first expressed as a GST fusion protein. The GST moiety was removed by thrombin digestion, and the resulting antigen was injected into rabbits. Antiserum was purified with CNBr-sepharose beads covalently coupled to the antigen.
wsp-1