Monoclonal antibodies against C. elegans SNB-1, without the transmembrane region were made by immunizing mice with a His6-tagged fusion protein to amino acids 1-86 which had previously been used for the generation of rabbit polyclonal antibodies. Three stable hybridoma cell lines (2E6, 5D1, and 5G1) were produced and all were of the isotype IgG1.
snb-1