Polyclonal rat anti-SYP-1 antibodies. The synthetic peptide ''DNFTIWVDAPTEALIETPVDDQS'' corresponding to the N-ter of C. elegans SYP-1 was used to immunize three Rats according to standard procedures (Genscript). After four rounds of immunization, the raw serum from each animal was pooled and affinity purified. Specificity of the antibody was assessed by immunofluorescence in WT worms subjected to syp-1RNAi in which, unlike in the untreated animals, SYP-1 loading was largely absent.
syp-1