WBAntibody00002907

Anti-LGG1 (Rabbit) antibodies were generated by Covalab (Vil- leurbanne, France) against peptides FEKRRAEGDKIRRKY and GQLYQDHHEEDLFLY (sequence optimized and kindly shared by Vincent Galy). Serum was immunopurified and anti-LGG-1 specificity was validated in western blots using WT, lgg-1 RNAi, and lgg-1 OE samples as controls lgg-1

WBAntibody00003039

Polyclonal rat anti-SYP-1 antibodies. The synthetic peptide ''DNFTIWVDAPTEALIETPVDDQS'' corresponding to the N-ter of C. elegans SYP-1 was used to immunize three Rats according to standard procedures (Genscript). After four rounds of immunization, the raw serum from each animal was pooled and affinity purified. Specificity of the antibody was assessed by immunofluorescence in WT worms subjected to syp-1RNAi in which, unlike in the untreated animals, SYP-1 loading was largely absent. syp-1