Rabbit anti-KLP-16 was generated by Covance using the N-terminal peptide sequence CMNVARRRSGLFRSTIGAPPK (synthesized by the Peptide Synthesis Core Facility at Northwestern University), then affinity purified and used at 1:2000. klp-16
Rat anti-AIR-2 was generated by Covance using the C-terminal peptide sequence KIRAEKQQKIEKEASLRNH (synthesized by the Peptide Synthesis Core Facility at North- western University), then affinity purified and used at 1:1000. air-2
The IMMT-1 antibody was generated by injecting His6-IMMT-1 into mice (BALB/c, 6 weeks postnatal, female) 3 times at 14-day intervals (250 μg of protein per injection) and used at 1:5,000. immt-1