A fragment of nrde-3 (nucleotides 1-1149, amino acids 1-383) was amplified from N2 cDNA and cloned into pSMT3 with SacI and XhoI restriction sites and transformed into BL-21 DE3 cells. 6xHIS-SUMO-NRDE-3 was purified in the same manner as 6xHIS-ENRI- 1. 6xHIS-SUMO-NRDE-3 was injected into rabbits for antibody production (Capralogics, Cambridge Massachusetts).
nrde-3