A full-length tra-1 cDNA (pDZ118) provided by Dr. David Zarkower (University of Minnesota, US) was used to express a 133 amino acid-long polypeptide (translated from the 14th exon of tra-1), which was subsequently used as epitope to generate rabbit polyclonal TRA-1 antibody. The antigen was expressed in QIAexpress system (Qiagen), and 6xHis tag was used for the purification after dialysis. The raised antibody specifically labels a 175-kDa protein.
tra-1