Polyclonal antibodies against an N-terminal fragment of UNC-16 (residues 1-506) were generated as follows: GST::UNC-16(1-506) was expressed from pGEX6P-1 in bacteria, purified as described below, and injected into rabbits (GeneCust). For affinity purifi- cation of the antibodies, the GST fusion protein was cleaved using Prescission protease, and UNC-16(1-506) was covalently coupled to a 1-ml HiTrap N-hydroxysuccinimide column (GE Healthcare).
unc-16