FAMK-1 (residues 60-512) was expressed using a modified pI- secSUMOstar vector (LifeSensors) and purified from Hi5 insect cells (Bac-to-Bac, B85502; Invitrogen; Xiao et al., 2013). The purified protein was sent to Cocalico Biologicals for immunizing rabbits. FAMK-1-specific antibodies were affinity purified from serum with a HiTrap NHS-activated affinity column (17-0716-01; GE Healthcare) on which FAMK-1 was immobilized.
famk-1