Mouse monoclonal antibody against recombinant protein. Anti-PAR-3 monoclonal antibody P4A1 was produced in collaboration with Ken Kemphues. Purified recombinant PAR-3 (see cgc2346) was injected into mice. Hybridoma supernatants were assayed by immunostaining early embryos fixed in bulk with paraformaldehyde and methanol. Antibody P4A1 stained early embryos in the same pattern as previously described PAR-3 polyclonal sera (Antibody: [cgc2346]:par-3) and did not stain either par-3(it71) or par-3(RNAi) early embryos.
par-3