Rabbit polyclonal antibody against SYP-2. The a-SYP-2 C-terminal antibody does not detect SYP-2 either on chromosomes in situ or on Westerns in either syp-2(me19) or syp-2(ok307) mutants, indicating that the antibody is specific to SYP-2. syp-2
Polyclonal rabbit antibody. Anti-IMA-2 antibodies were affinity purified on full-length IMA-2 S peptide-tagged fusion proteins immobilized on polyvinylidene difluoride membranes. The specificity of the affinity-purified antibodies was determined by immunoblotting whole worm lysates and bacterial lysates containing expressed recombinant IMA proteins. ima-2
Rabbit polyclonal antibody against CUT-2. Rabbits were immunized by cuticlin residue, no details on antibody purification was mentioned in original publication. cut-2
Polyclonal rabbit antibodies against recombinant APR-1 protein. Antisera were affinity purified against the recombinant APR-1 fragment immobilized on PVDF membranes (Immobilon). apr-1
Polyclonal rat antibodies against recombinant APR-1 protein. Antisera were affinity purified against the recombinant APR-1 fragment immobilized on PVDF membranes (Immobilon). apr-1
Monoclonal antibody M44 is a mouse IgG1, generated by immunization with C. elegans homogenates, that reacts with a cell surface antigen on the PVP neurons.
A GST fusion protein of LMW-PTP (GST-LMW-PTP; Y94H6A.7) was purified from an E. coli expression system harboring the pGex-2T vector. The pu- rified protein was used to immunize a rabbit to generate anti-serum. The antibody was then purified through negative selection on GST-aga- rose and subsequently positive selection on GST-LMW-PTP-agarose. Y94H6A.7
Rabbit polyclonal antibodies were generated by injection of the C-terminus of RIC-3 fused to GST, the resulting serum was affinity purified on Affigel 15 (Shteingauz et al., 2009). ric-3
Rabbit polyclonal anti-ZIPT-7.1 antibodies were generated and purified by YenZyme. Two peptides based on the predicted ZIPT-7.1 protein sequence were used as antigens (#68-82, TSHREIQHSRLSTLK, and #138-150, SLSPHDHSHDHHD). zipt-7.1
Polyclonal rabbit antibody. Antibodies were raised against a His6-tagged fusion to amino acids 1-142 of UNC-10. The fusion protein was purified on Ni-NTA agarose in 8M urea, and renatured by dialysis against phosphate buffered saline. unc-10