Affinity purified rabbit anti-WHT-2 antibodies were generated against cys-321EKSDEGQRPSERHK or cys-599YAGLALRVSKAR peptides by Bethyl Laboratories (Montgomery, TX). Both antisera stained gut granules in C. elegans embryos, however the WHT-2(321) antibody exhibited less non-specific signal than the WHT-2(599) antibody, and therefore was used for the studies presented.
wht-2