For NTL-1, the region corresponding to amino acid position 650-950 was amplified from cDNA using forward primer: 5'-ATAATAGGATCCAGGTAATGAAAGAGAACTCGG-3' (Tdo1707); and reverse primer: 5' -TATTATGGATCCCAAATTTTCCACTGACATCGC-3' (Tdo1708) and cloned into pCAL-KC via BamHI/BamHI. This construct was used as a template for generating the antigen for mouse polyclonal against NTL-1 (4162, Capralogics Inc.).
let-711