Anti eUNC-119N antibodies were raised against the synthetic peptide QQSIAPGSATFPSQMPRGGC. These peptides were conjugated to Keyhole limpet hemocyanin. The specific IgG was affinity purified using antigenic peptides that were conjugated to activated thiol- Sepharose 4B (GE healthcare Bio-Science KK, Tokyo, Japan) according to the manufacturer's instruction.
unc-119