Affinity-purified rabbit polyclonal antibodies against an N-terminal region of LIS-1 (residues 1 - 141) were generated as described previously (Desai et al., 2003). In brief, GST::LIS-11-141 was expressed from pGEX6P1 in Escherichia coli, purified using glutathione resin (Thermo Scientific), and injected into rabbits (GeneCust). Serum was affinity purified on a HiTrap N-hydroxysuccinimide column (GE Healthcare) against covalently coupled LIS-11-141.
lis-1