Mouse monoclonal antibody against UNC-1 recombinant protein. Antibodies to UNC-1 were prepared in the laboratory of Man Sun Sy. Two mice were inoculated three times with UNC-1 protein. Once a response was documented from the serum of the mice, hybridomas were made and screened for anti-UNC-1. Seven monoclonal antibodies reacting with UNC-1 were isolated. Each antibody was screened for the ability to bind UNC-1 on a Western blot and for immunocytochemical staining in N2, as well as loss of staining in the unc-1 null alleles fc53 and e580. Five monoclonal antibodies specific for UNC-1 were isolated, each of which gave identical binding in nematodes and on Western blots. One monoclonal antibody, 5D5, was used for all the studies.
unc-1