The monoclonal antibody against C. elegans HMR-1 was made by immunizing mice with a His6-tagged fusion protein encompassing amino acids: 1099-1223. Two stable hybridoma cell lines, 8H10 and 1A7, were produced. The 8H10 monoclonal was the isotype IgM and the 1A7 monoclonal was a mixed isotype of IgG1+IgG2a+IgM.
hmr-1