Human SPOP is highly homologous to C. elegans SPOP-1 with 65.9% amino acid homology. We raised monoclonal antibodies against human SPOP by producing recombinant SPOP as a 6×His tagged protein in E. coli and used the purified full length SPOP protein as an immunogen to inject naïve BALB/C mice. All mAb production work was conducted at the Washington State University Monoclonal Antibody Center as a fee for service project. Human SPOP reactive mAb clones were screened for reactivity against C. elegans SPOP-1 protein, and clone 8B was selected for its specificity.
spop-1