Rabbit polyclonal antibody raised against MBPGPB-1 and GSTGPB-1 fusion proteins. The antiserum 68088 used in this study specifically recognized a band of approximately 36 kDa in wild-type N2 extracts, the typical size of G protein subunits. In extracts of a gpb-1 deletion strain rescued by the extrachromosomal transgene (NL361), the same band was detected, indicating that the transgene produced a GPB-1 protein of normal size. The antibody has virtually no cross reactivity, as assayed by in situ immunohistochemistry on gpb-1 embryos.
gpb-1