The polyclonal antibodies aDEB-1/a*e and aDEB-1/ b*f were produced by Clonestar Peptide Service (Brno, Czech Republic) using synthetic peptides of the fol- lowing sequences: DEB-1/a*c: CRKLADRLNPQDR, and DEB-1/b*f: VSDHYDSSDEYD. deb-1
The polyclonal antibodies aDEB-1/a*e and aDEB-1/ b*f were produced by Clonestar Peptide Service (Brno, Czech Republic) using synthetic peptides of the fol- lowing sequences: DEB-1/a*c: CRKLADRLNPQDR, and DEB-1/b*f: VSDHYDSSDEYD. deb-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). csc-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). bub-1