Affinity-purified rabbit polyclonal antibodies against the C-terminal region of DNC-1 (residues 1,037-1,351) were generated as described previously (Desai et al., 2003). In brief, GST fusions were expressed in Escherichia coli, purified, and injected into rabbits (Co- vance). Serum was affinity-purified sequentially against coupled GST followed by coupled GST::DNC-1.
dnc-1