The monoclonal ASH-2 antibody was generated by injection of a fusion protein between GST and amino acids 285-572 of C. elegans ASH-2 and was affinity-purified by Abmart (Shanghai, China). The H3K4me3 antibody (8580), the H3K4me1 antibody (8895), the H3K9me3 antibody (8898) and the H3 antibody (1791) were obtained from Abcam. The H3K4me2 antibody (07-030) was obtained from Upstate Biotechnology.
ash-2