The anti-GLD-2 affinity-purified serum rt317 was generated by immunizing rats with a GLD-2(aa959-1113) glutathione S-transferase- fusion protein expressed and purified as described. gld-2
The rabbit anti-GLD-2 antibody used in the immunoprecipitation experiments was generated by immunizing New Zealand White rabbits with an epitope that covered amino acid 959 to 1113 of GLD-2, fused to a GST-affinity tag. gld-2
Polyclonal rabbit antibody. Polyclonal antibodies were generated from rabbits using a keyhole limpet haemocyanin (KLH)-conjugated peptide corresponding to GLD-2 amino acids 108 to 127. gld-2