The goal of this experiment was to identify transcripts regulated non-autonomously by
age-1 activity in neurons or intestine. Comparisons of gene expression were made between
age-1(
mg44) null adults and CY251 (
age-1(
mg44); bvIs2) and CY262 (
age-1(
mg44); bvIs1); all were relative to wildtype (N2). The integrated transgenes, bvIs2 and bvIs1, carry the
age-1 cDNA expressed from the neuronal
ric-19 promoter (bvIs2) or intestinal
ges-1 promoter (bvIs1). Transcripts upregulated in
age-1(
mg44) adults compared to wildtype (>=1.5 fold; p<0.05) or downregulated (<0.6 fold; p<0.05) were considered to be
age-1 target genes. Rescue in CY251 or CY262 was determined by change in the transgenic background versus
age-1(
mg44) (all relative to N2 wildtype). Non-autonomous rescue of a target gene was usually determined by transcript levels that were at least 50% rescued compared to wildtype.