We have shown that worms that possess mutations in two electron transport chain subunits live longer due to mtROS signalling. Wild type worms can also live longer by treatment with the pro-oxidant paraquat. Paraquat treatment is not additive to the mutations in the ETC subunits. We aimed to determine the underlying changes in gene expression that were common to both paraquat treatment and the two mutations. We also have shown genetically that the mtROS signal that leads to extended lifespan is dependent on CED-4. We generated double mutants that lack functional CED-4 with the two mutations in the ETC subunits. We have found that a large number of gene expression changes by mtROS signalling are reverted by loss of CED-4.
Illumina Genome Analyzer II paired end sequencing; Illumina sequencing of C. elegans dauer exit daf-2(el370) sample 2 DauerExitDAF2-2 polyA+ RNAseq random fragment library RW0001
Illumina Genome Analyzer II paired end sequencing; Illumina sequencing of C. elegans dauer entry daf-2(el370) sample 2 DauerEntryDAF2-2 polyA+ RNAseq random fragment library RW0001
Illumina Genome Analyzer II paired end sequencing; Illumina sequencing of C. elegans dauer daf-2(el370) sample 2 DauerDAF2-2 polyA+ 76bp SE RNAseq random fragment library RW0001
Illumina HiSeq 2000 paired end sequencing; Illumina sequencing of C. elegans dauer daf-2(el370) sample 2-1 DauerDAF2-2-1 polyA+ 100bp PE RNAseq random fragment library RW0001