We found that the cell type that expressed the transgene most robustly at every developmental stage is the canal-associated neurons (CANs), whose cell body are near the midbody with processes that run along the excretory canal laterally along most of the body. All developmental stages also show
Ppa-hsd-2p::rfp expression in a few intestinal cells as well as the excretory gland cell, though expression in the excretory canal was detectable only in the dauer stage.