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WormBase Tree Display for Microarray_experiment: [cgc5303]:SL2_enriched

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Name Class

[cgc5303]:SL2_enrichedMicroarraySMD2
SpeciesCaenorhabditis elegans
SampleSample_A[cgc5303]:SL2_enriched
Sample_B[cgc5303]:poly_A
ReferenceWBPaper00005303
RemarkCy3-dUTP was used to label SL2-enriched cDNA and Cy5-dUTP was used to label cDNA from poly(A)+ RNA made from a mixed stage population of wild-type worms. The SL2-enriched probe and the probe from the starting poly(A)+ mRNA were simultaneously hybridized to DNA microarrays. To ensure reproducibility, this procedure was repeated five times. Ratios of Cy3/Cy5 (SL2/poly(A)+) signals were calculated for each gene and converted to log2(ratio). The average log2(ratio) from the five repeats was then calculated.