Phenotypes:

Alleles for which the sequence change is known are listed in boldface.

The following phenotypes have been observed in unc-2:

Phenotype Entities Affected Supporting Evidence
locomotion variantAllele:
lj1
Curator: Carol Bastiani
Person evidence: William Ronald Schafer
Recessive:
Variation effect: Null; Person_evidence; Curator_confirmed
details
cell physiology phenotypeAllele:
e55
Curator: Karen Yook
Paper evidence: Stout RF & Parpura V, 2011
Remark: Cultured CEPsh glial cells from mutant worms exhibit altered intracellular calcium-based responses to depolarization.
details
path curvature increasedAllele:
locomotion variantAllele:
mu74
Curator: Carol Bastiani
Person evidence: William Ronald Schafer
Recessive:
Variation effect: Null; Person_evidence; Curator_confirmed
details
synapse morphology variant Allele:
e55
Curator: Brian Ackley
Paper evidence: Caylor RC, Jin Y & Ackley BD, 2013
Recessive:
Remark: GABAergic neuromuscular junctions (NMJs) are enlarged
Variation effect: Probable null via phenotype; Curator_confirmed; Paper_evidence
details
hyperactive egg layingAllele:
ra612
Curator: Carol Bastiani
Person evidence: Colin Thacker
details
backing decreasedAllele:
tail bend angle increasedAllele:
backward point velocity variantAllele:
hyperactive egg layingAllele:
ra606
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
sluggishAllele:
mu74
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: resembles e55
details
thinAllele:
serotonin hypersensitiveAllele:
e55
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: hypersensitive to serotonin
details
hyperactive egg layingAllele:
mu74
Curator: Carol Bastiani
Person evidence: William Ronald Schafer
Recessive:
Variation effect: Null; Person_evidence; Curator_confirmed
details
protein expression absentAllele:
e55
Affected by molecule: isoamyl alcohol
Curator: Karen Yook
Paper evidence: Hirotsu T, Saeki S, Yamamoto M & Iino Y, 2000
Remark: No staining of activated MAP kinase was observed, even in after odorant stimulus, whereas immunoreactivity against the anti-activated-MAPK antibody was observed in the AWC neurons (and others) after 10 seconds of application with isoamylalcohol.
Variation effect: Probable null via phenotype; Curator_confirmed; Paper_evidence
details
axon branching variantAllele:
e55
Curator: Gary C. Schindelman
Paper evidence: Chen CH, Lee A, Liao CP, Liu YW & Pan CL, 2014
Remark: Branch defects scored in PLM neuron.
details
pausing variantAllele:
thinAllele:
e55
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
details
dopamine adaptation variantAllele:
e55
Affected by molecule: dopamine
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: Fails to desensitize to dopamine.
details
amplitude of sinusoidal movement variantAllele:
hyperactive egg layingAllele:
e55
details
locomotion variantAllele:
ra606
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
forward locomotion decreasedAllele:
loss of asymmetry AWCanatomy-term: AWC, abnormalAllele:
e55
Curator: Jolene S. Fernandes; Karen Yook
Paper evidence: Troemel ER, Sagasti A & Bargmann CI, 1999; Chang C, Hsieh YW, Lesch BJ, Bargmann CI & Chuang CF, 2011
Remark: Loss-of-function mutants showed a 2AWC-ON phenotype resembling that caused by nocodazole treatment.; Mutants are defective in str-2 asymmetry
Variation effect: Probable null via phenotype; Curator_confirmed; Paper_evidence
details
dopamine adaptation variantAllele:
mu74
Affected by molecule: dopamine
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: resembles e55
details
locomotion variantAllele:
ra612
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
defecation variantAllele:
e55
details
pharyngeal pumping irregularanatomy-term: pharynx, abnormalAllele:
e55
Curator: Jolene S. Fernandes
Paper evidence: Avery L, 1993
details
defecation variantAllele:
ra612
Curator: Carol Bastiani
Person evidence: Colin Thacker
details
thinAllele:
mu74
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: resembles e55
details
serotonin hypersensitiveAllele:
mu74
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: resembles e55
details
head bend angle variantAllele:
defecation variantAllele:
ra606
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
body posture amplitude decreasedAllele:
coiling frequency increasedAllele:
kinkerAllele:
e55
Curator: Jolene S. Fernandes; Karen Yook
Ease of scoring: Es3 easy to score
Paper evidence: Avery L, 1993
Person evidence: Jonathan Hodgkin
Remark: weak kinker
details
loss of left right asymmetry
  • anatomy-term: AWC-OFF, abnormal
  • anatomy-term: AWC, abnormal
  • anatomy-term: AWC-ON, abnormal
Allele:
e55
Curator: Karen Yook
Paper evidence: Chuang CF, VanHoven MK, Fetter RD, Verselis VK & Bargmann CI, 2007
Remark: Animals express str-2::GFP in both AWC cells, e.g. both AWC neurons are AWC on , whereas other animals have AWC symmetry similar to wild-type and a few animals have are 2 AWC off (n=103).
Variation effect: Probable null via phenotype; Curator_confirmed; Paper_evidence
details
locomotion variantAllele:
ra609
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
hyperactive egg layingAllele:
lj1
Curator: Carol Bastiani
Person evidence: William Ronald Schafer
Recessive:
Variation effect: Null; Person_evidence; Curator_confirmed
details
nose movement variantAllele:
body posture wavelength decreasedAllele:
frequency of body bend variantAllele:
sluggishAllele:
e55
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
details
forward point velocity variantAllele:
roaming reducedAllele:
shortAllele:
hyperactive egg layingAllele:
ra609
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
pharyngeal pumping reducedanatomy-term: pharynx, abnormalAllele:
e55
Curator: Jolene S. Fernandes
Paper evidence: Avery L, 1993
details
defecation variantAllele:
ra609
Curator: Carol Bastiani
Person evidence: Colin Thacker
Recessive:
details
kinkerAllele:
mu74
Curator: Karen Yook
Person evidence: Jonathan Hodgkin
Remark: resembles e55
details
locomotion variantAllele:
e55
Curator: Carol Bastiani
Paper evidence: Brenner S, 1974
Person evidence: National Bioresource Project c/o Dr. Shohei Mitani
Recessive:
details
turning frequency variantAllele:
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059972
Genotype: unc-43(n498n1186)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 4 out of 4 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059971
Genotype: goa-1(sa734)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 3 out of 4 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059966
Genotype: eri-1(mg366); dgk-1(nu62) lin-15B(n744)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 8 out of 8 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059968
Genotype: eri-1(mg366); dgk-1(nu62) lin-15B(n744)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 4 out of 4 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059965
Genotype: eri-1(mg366); lin-15B(n744)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 6 out of 8 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059970
Genotype: eat-16(sa768)
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 4 out of 4 trials.
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059969
Paper evidence: Sieburth D et al., 2005
Remark: Animals displayed resistance to paralysis on aldicarb in 4 out of 4 trials.
details

Phenotype not observed:
The following phenotypes have been reported as NOT observed in unc-2
Phenotype Entities Affected Supporting Evidence
fat content variantAllele:
e55
Curator: Jolene S. Fernandes
Paper evidence: Srinivasan S et al., 2008
Remark: Mutants had wild-type fat content
Treatment: Fat content was visualized by Nile red staining
details
protein degradation variantAllele:
e55
Curator: Chris Grove
Paper evidence: Shephard F, Adenle AA, Jacobson LA & Szewczyk NJ, 2011
Remark: Table S2
details
serotonin induced fat reduction variantAllele:
e55
Curator: Jolene S. Fernandes
Paper evidence: Srinivasan S et al., 2008
Remark: Mutants were susceptible to the fat-reducing effects of exogenously administered serotonin
Treatment: Reduced fat content of 5-HT-treated animals was visualized by Nile red staining and confirmed by thin-layer chromatography (TLC) quantitation of total triglycerides extracted from vehicle- and 5-HT-treated worms and by Sudan black fat staining
details
loss of asymmetry ASEAllele:
mu74
Curator: Jolene S. Fernandes
Paper evidence: Chang S, Johnston RJ & Hobert O, 2003
Remark: No disruption of ASE asymmetry (as seen with lim-6 reporters)
Variation effect: Null; Curator_confirmed; Paper_evidence
details
lethalAllele:
tm10264
Curator: Karen Yook
Person evidence: National Bioresource Project c/o Dr. Shohei Mitani
Remark: Classified as homozygous viable by the National BioResource Project of Japan.
details
loss of asymmetry ASEAllele:
e55
Curator: Jolene S. Fernandes
Paper evidence: Chang S, Johnston RJ & Hobert O, 2003
Remark: No disruption of ASE asymmetry (as seen with lim-6 and gcy-7 reporters)
Variation effect: Hypomorph reduction of function; Curator_confirmed; Paper_evidence
details
lethalAllele:
tm8311
Curator: Karen Yook
Person evidence: National Bioresource Project c/o Dr. Shohei Mitani
Remark: Classified as homozygous viable by the National BioResource Project of Japan.
details
larval lethalRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
exploded through vulvaRNAi:
maternal sterileRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
postembryonic development variantRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
nematode phenotypeRNAi:
WBRNAi00052199
Paper evidence: Sonnichsen B et al., 2005
details
larval arrestRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
body wall muscle sarcomere morphology variantanatomy-term: body wall muscle cell, abnormalRNAi:
WBRNAi00090152
Genotype: jIs01 [myo-3::GFP], translational fusion
Paper evidence: Shephard F, Adenle AA, Jacobson LA & Szewczyk NJ, 2011
details
nematode phenotypeRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
mitochondria morphology variantanatomy-term: body wall muscle cell, abnormalRNAi:
WBRNAi00090311
Genotype: ccIs4251 [Pmyo-3::MitGFP, Pmyo-3::NLS::GFP-lacZ]
Paper evidence: Shephard F, Adenle AA, Jacobson LA & Szewczyk NJ, 2011
details
sterile progenyRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
slow growthRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
nematode phenotypeRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
larval lethalRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
slow growthRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
embryonic lethalRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059973
Paper evidence: Sieburth D et al., 2005
details
protruding vulvaRNAi:
organism morphology variantRNAi:
organism morphology variantRNAi:
WBRNAi00052199
Paper evidence: Sonnichsen B et al., 2005
details
postembryonic development variantRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
maternal sterileRNAi:
WBRNAi00052200
Paper evidence: Sonnichsen B et al., 2005
details
postembryonic development variantRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
protein degradation variantRNAi:
WBRNAi00089993
Genotype: ccIs55 [unc-54::lacZ]
Paper evidence: Shephard F, Adenle AA, Jacobson LA & Szewczyk NJ, 2011
details
larval arrestRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
egg laying defectiveRNAi:
maternal sterileRNAi:
WBRNAi00052199
Paper evidence: Sonnichsen B et al., 2005
details
sterile progenyRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
sterileRNAi:
slow growthRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
nematode phenotypeRNAi:
WBRNAi00052200
Paper evidence: Sonnichsen B et al., 2005
details
embryonic lethalRNAi:
WBRNAi00052200
Paper evidence: Sonnichsen B et al., 2005
details
larval arrestRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
nematode phenotypeRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
growth rate variantRNAi:
rollerRNAi:
maternal sterileRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
embryonic lethalRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
embryonic lethalRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
dumpyRNAi:
locomotion variantRNAi:
larval lethalRNAi:
WBRNAi00013890
Paper evidence: Kamath RS et al., 2003
details
organism morphology variantRNAi:
WBRNAi00052200
Paper evidence: Sonnichsen B et al., 2005
details
maternal sterileRNAi:
WBRNAi00022432
Paper evidence: Kamath RS et al., 2003
details
aldicarb resistantlife-stage: adult Ce, abnormalRNAi:
WBRNAi00059967
Genotype: eri-1(mg366); lin-15B(n744)
Paper evidence: Sieburth D et al., 2005
details
sterile progenyRNAi:
WBRNAi00018019
Paper evidence: Kamath RS et al., 2003
details
embryonic lethalRNAi:
WBRNAi00052199
Paper evidence: Sonnichsen B et al., 2005
details

Interaction-based phenotypes:

The following phenotypes are indirectly caused or affected by some perturbation of unc-2 in the context of a genetic interaction :

Phenotype Interactions Interaction Type Citations
loss of asymmetry AWC Epistasis
loss of asymmetry AWC Masking

Overexpression:
Overexpression of the unc-2 gene results in the following phenotypes.