Figure 4. FIGL-1 activity is vital for C. elegans embryos in the absence of a functional spindle-assembly checkpoint. (A) The localization of FIGL-1 and MEL-26 was determined by immunofluorescence with affinity-purified antibodies in wild-type and
figl-1(RNAi) early embryos. The DNA was visualized by Hoechst staining (right panels). Bar, 10 um. (B,C) L3 larvae were injected with double-stranded RNA against
figl-1 or the mitotic checkpoint components
san-1 (S. cerevisiae MAD3),
mdf-1 (S. cerevisiae MAD1) or Y54G9A.6 (S. cerevisiae BUB3) either alone or in combination. Embryonic lethality was scored after the times indicated and plotted (24-48 hours). The values obtained after 24 and 48 hours of inactivation are listed in panel C, and schematically presented in panel B.