(B) Coexpression of alpha-tubulin and beta-tubulin TagRFP-T protein fusions and uIs31 TRN::GFP transcriptional fusion. Left, uIs31(
mec-17p::gfp) showing PLM. Center, TagRFP-T tubulin fusion protein. Right, composite image. TagRFP-T was false colored as magenta for enhanced visibility. Expression of isoforms inTRNs is seen as white in the composite overlap of uIs31 marker and TagRFP-T::Tubulin. Images are oriented so that the anterior of the animal is on the left and the ventral side is on the bottom. Scale bar, 50 um.