Fig. 1-Comparison between amino acid sequences of C. elegans mtSSB1, other mtSSBs, and bacterial SSBs and expression analyses of the mtssb-1 gene. (A) The tree was derived using CLUSTALW and TreeView software from comparisons between the amino acid sequences of the following putative and known mtSSB and bacterial SSB proteins: PAR2.1/mtSSB1 (WP:CE00768), C. briggsae BP: CBP07509 (CBG06914), human SSBP1 (ENSP00000265304), Drosophila mtSSB-PA (CG4337: FBgn0010438), B. bacteriovorus SSB (Q1IPH6-ACIBL), and X. fastidiosa SSB1 (Q9PHE7). (B) The expression levels of mtssb-1 during development weremonitored with real-time quantitative RT-PCR as compared with the expression level of an elongation factor
eft-2. Relative ratio of each expression was calculated using the adult expression levels. (C) Real-time quantitative RT-PCR analysis for expression of mtssb-1 gene in the mtssb-1 RNAi and in the ethidium bromide treatment. The
eft-2 expression level was used as an internal standard. Relative ratio of each gene expression in the samples, RNAi vector feeding control vs. mtssb-1 RNAi or N2 wild-type control vs. ethidium bromide treatment was calculated using the higher expression levels among each gene set. Triplicate real-time RT-PCR experiments in each sample were carried out. Vertical bars indicate standard error. ** Shows statisticalsignificance at 0.01 level (Student's t test).