Figure S2. The
daf-7 smFISH Neuronal Expression Pattern Is Specific and Includes the OLQ and ADE Mechanosensory Neurons, Related to Figure 2 (A)
daf-7 single molecule FISH in
daf-7(
ok3125) animals carrying a 664 bp deletion in the
daf-7 gene. Nonspecific fluorescence is restricted to muscle tissue. (B-D)
daf-7 is expressed in the OLQ neurons (white arrows).
daf-7 smFISH (B),
ocr-4::gfp in OLQ neurons (Tobin et al., 2002) (C), and merge (D). (E-F)
daf-7 is expressed in the ADE neurons (white arrow).
daf-7 smFISH (E),
cat-2::gfp in ADE neurons (and other neurons) (Flames and Hobert, 2009) (F), andmerge (G).