Figure 1. Survey for novel target genes of the terminal selector UNC-3: UNC-3 ChIP-Seq signal is aligned to specific gene loci of interest. Reporter constructs carrying the genomic region bound by UNC-3 followed by tagRFP are used to build transgenic animals, and the expression images are shown (scale bar: 50 μm). A-F: Six reporters are expressed in MNs and their
unc-3 dependency is assessed. The expression of fluorescent reporters is imaged and compared between wildtype and
unc-3 (
n3435) mutant animals. The number of ventral nerve cord MNs showing tagRFP expression is quantified for each genotype (N=10) and shown in boxplots. Student t test is performed and statistical significance is determined by p-value. Three reporter genes (
nep-21, D2007.2,
dmsr-2) show decrease of expression in
unc-3 (
n3435) mutants (A-C), while the remaining three genes (
ncs-2,
npr-29,
drn-1) do not show changes in their expression (D-F). G: Four reporter genes (
pxd-1,
cal-2,
lgc-4, and
ldb-1) are not expressed in MNs of the ventral cord but are expressed in head neurons (all 4 genes), tail neurons (
pxd-1 and
cal-2), and muscle or epidermal cells (
pxd-1 and
cal-2). Zoomed insets are shown on the right of each image.