Figure 5. in vivo expression of a subset of genes with different RNAi phenotypes. C03D6.1, K04G7.1, C27F2.7, and
sac-1 had strongly different RNAi phenotypes in C. elegans and C. briggsae. We generated transgenic C. elegans strains (N2) expressing GFP under control of the promoter of the C. elegans orthologue or C. briggsae strains (AF16) expressing mWormCherry under control of the orthologous C. briggsae promoter for each gene. In each case, four panels are shown: DIC image of N2 worms transgenic for the C. elegans promoter driving GFP, fluorescence image of N2 worms transgenic for the C. elegans promoter driving GFP, DIC image of AF16 worms transgenic for the C. briggsae promoter driving mWormCherry expression, fluorescence image of AF16 worms transgenic for the C. briggsae promoter driving mWormCherry expression. Images are confocal projections at 2006magnification, and scale bars represent 100 mm, except for C. elegans K04G7.1 which is at 4006magnification with a scale bar representing 50 um. Images are representative of 3 independent lines. A. Expression difference for C03D6.1. Arrow heads indicate tail cells (white), intestine (red) and hypodermis (blue). B. Expression difference for K04G7.1. Arrow heads indicate head neurons (white) and body wall muscle (red). C. Expression difference for C27F2.7. Arrow heads indicate head neurons (white), hypodermis (blue), intestine (yellow), vulva (green) and tail neurons (red). D. Expression difference for
sac-1. Shown are 3 confocal projections along the body of the animals 4006magnification. Scale bars represent 50 um. Arrowheads indicate the intestine (blue), pharynx and pharyngeal neurons (white), spermatheca (yellow), and tail cells (red).