"To validate the RNAi approach, we used mutants of three highly similar nuclear hormone receptor (NHR) paralogs,
nhr-68,
nhr-101, and
nhr-114, which have identical regulatory interaction profiles in the GRN (Table S2). We crossed each mutation into reporter strains where GFP expression is driven by the
acdh-1 promoter (Pacdh-1::GFP) or the
acdh-2 promoter (Pacdh-2::GFP) and observed very similar effects as we did by RNAi - namely, decreased fluorescence of Pacdh-1::GFP and increased fluorescence of Pacdh2::GFP, in the intestine (Figure 2B)."